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KMID : 0613820170270091070
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Journal of Life Science
2017 Volume.27 No. 9 p.1070 ~ p.1077
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Cytoprotective Effects of Schisandrin A against Hydrogen Peroxide-induced Oxidative Stress in SW1353 Human Chondrocytes
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Jeong Jin-Woo
Choi Eun-Ok
Kwon Da-Hye
Kim Bum-Hoi
Park Dong-Il
Hwang Hye-Jin
Kim Byung-Woo
Choi Yung-Hyun
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Abstract
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Chondrocyte apoptosis induced by reactive oxygen species (ROS) plays an important role in the pathogenesis of osteoarthritis. Schisandrin A, a bioactive compound found in fruits of the Schisandra genus, has been reported to possess multiple pharmacological and therapeutic properties. Although several studies have described the antioxidant effects of analogues of schisandrin A, the underlying molecular mechanisms of this bioactive compound remain largely unresolved. The present study investigated the cytoprotective effect of schisandrin A against oxidative stress (hydrogen peroxide [H2O2]) in SW1353 human chondrocyte cells. The results showed that schisandrin A preconditioning significantly inhibited H2O2-induced growth inhibition and apoptotic cell death by blocking the degradation of poly (ADP-ribose) polymerase proteins and down-regulating pro-caspase-3. These antiapoptotic effects of schisandrin A were associated with attenuation of mitochondrial dysfunction and normalization of expression changes of proapoptotic Bax and antiapoptotic Bcl-2 in H2O2-stimulated SW1353 chondrocytes. Furthermore, schisandrin A effectively abrogated H2O2-induced intracellular ROS accumulation and phosphorylation of histone H2AX at serine 139, a widely used marker of DNA damage. Thus, the present study demonstrates that schisandrin A provides protection against H2O2-induced apoptosis and DNA damage in SW1353 chondrocytes, possibly by prevention of ROS generation. Collectively, our data indicate that schisandrin A has therapeutic potential in the treatment of oxidative disorders caused by overproduction of ROS.
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KEYWORD
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Antioxidant, apoptosis, DNA damage, ROS, schisandrin A
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